As each LeucoPatch contains a substantial number of leucocytes, the aim of the study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the LeucoPatch. The researchers describe how they used a burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescent in situ hybridization (FISH) assay to show significant respiratory burst in PMNs and active phagocytosis and killing of Pseudomonas aeruginosa by the LeucoPatch. In addition, it was shown that PMNs from LeucoPatch migrate toward, as well as phagocytise, Pseudomonas aeruginosa. The study substantiated that the beneficial clinical effect in chronic wounds by LeucoPatches is in part attributed to the activity of the polymorphonuclear neutrophils (PMNs) in the LeucoPatch.
Details of the paper are: THE PHAGOCYTIC FITNESS OF LEUCOPATCHES MAY IMPACT THE HEALING OF CHRONIC WOUNDS Authors: Thomsen, Kim; Trøstrup, Hannah; Christophersen, Lars; Lundquist, Rasmus; Høiby, Niels; Moser, C Manuscript ID: CEI-2015-5672.R1
The abstract for the paper can be found at: onlinelibrary.wiley.com/doi/10.1111/cei.12773/abstract